Studies initially focused on HHV-8 and its association with KS, transmission of HHV-8, and the natural history of HHV-8 infection. HHV-8 DNA has been detected in both tissues and bodily fluids. Since then it also has been associated with the development of multicentric Castleman's disease and primary effusion lymphoma (PEL). Also known as Kaposi's sarcoma associated herpes virus, HHV-8 was first discovered in 1994 in KS-affected tissue. Homosexual men infected with HIV-1 are at increased risk for developing AIDS-related Kaposi's sarcoma (AIDS-KS) due to co-infection with HHV-8, a gamma herpes virus. These real-time NASBA assays with beacon detection provide tools for further study of HHV-8 expression in patient material. We found HHV-8 mRNA expression in 9 out of the 10 tested samples. The linear dynamic range was 50 to 10 7 molecules of HHV-8 mRNA. Resultsįor all four assays, the limit of detection (LOD) of 50 molecules and the limit of quantification (LOQ) of 100 molecules were determined using in vitro transcribed RNA. The assays were tested on PBMC samples of two AIDS-KS patients from the Amsterdam Cohort. A molecular beacon is used during amplification to enable real-time detection of the product. The NASBA technique amplifies nucleic acids without thermocycling and mRNA can be amplified in a dsDNA background. We have developed four quantitative nucleic acid sequence-based amplification assays (NASBA-QT) specifically to detect mRNA coding for ORF 73 (latency-associated nuclear antigen, LANA), vGCR (a membrane receptor), vBcl-2 (a viral inhibitor of apoptosis) and vIL-6 (a viral growth factor). To examine the expression of HHV-8 in PBMC, four HHV-8 mRNA specific NASBA assays were developed Methods Endothelial dysfunction is the major cause of vascular complications in diabetes.Human herpesvirus-8 (HHV-8) is linked to the pathogenesis of Kaposi's sarcoma (KS), and the HHV-8 DNA load in peripheral blood mononuclear cells (PBMC) is associated with the clinical stage of KS. is traditionally used for the production of antidiabetic herbal medicine. polium hydroalcoholic extract on the vasoreactivity and endothelial nitric oxide synthase (eNOS) and vascular cell adhesion molecule (VCAM)-1 genes expression as well in streptozotocin (STZ)-induced diabetic rat aorta.Īs a result of this, the present study was conducted to evaluate the impacts of T. Male Wistar rats were randomly divided into six groups: control, diabetic, metformin, and three groups of T. The control and diabetic groups were given normal saline metformin group was given 300 mg/kg metformin and T. polium groups were given 100, 200, and 400 mg/kg T. polium extract, daily by gavage for 6 weeks. polium extract was found to significantly reduce serum glucose level. It was also observed that metformin and T. Polium extract significantly improved vasorelaxant response of aortic rings to acetylcholine (Ach). Real-time polymerase chain reaction (PCR) analysis showed that T. polium and metformin significantly increased eNOS expression, while it decreased VCAM-1 expressions in aortic tissue of diabetic rats. polium extract could improve endothelial dysfunction by ameliorating the vasoreactivity and regulating eNOS and VCAM-1 gene expressions as well in STZ-induced diabetic rats' aorta.ĭiabetes is a metabolic disorder with the known main characteristic of hyperglycemia and glucose intolerance due to insulin deficiency, tissue resistance to insulin or both. It is a known risk factor for atherosclerosis and cardiovascular diseases. Atherosclerosis is a complicated and multi-faceted process of disease where endothelial dysfunction, formation of foam cell, retention of lipoprotein, oxidative stress, and inflammation cause plaque formation and progression, aggregation of platelet, plaque rupture, and formation of thrombus.
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